Publications
2025
- In Vitro Characterization of the Aromatic SAM-Dependent C-Methyltransferase NapB5; Breiltgens, J.; Paul, A.; Zou, Z.; Andexer, J. N.; Müller, M.; J. Nat. Prod. 2025.
Manuscript and Research Data - Chimeric cofactors enable methyltransferase-catalyzed prenylation;
- Biocatalytic Alkylation of Ambident Nucleophiles Enables Selective N-Functionalization of Heterocycles and Late-Stage Modifications; Ospina, F.; Schülke, K. H.; Schnutenhaus, M.; Klein, A.; Desai, O.; Jain, S.; Krofta, C.; Stratmann, L.; Yang, J.; Gröger, H.; Hammer, S. C.; Angew. Chem. Int. Ed. 2025, 64, e202510300.
Manuscript and Research Data
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Efficient Transferase Engineering for SAM Analog Synthesis Using Combinatorial Library Design and High-Throughput LC/MS Screening; Bocquin, L.; Reese, F.; Schnutenhaus, M.; Hammer, S. C.; Methods Enzymol., 2025. in press doi.org/10.1016/bs.mie.2025.08.020.
Book Chapter
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How to Tell an N from an O: Controlling the Chemoselectivity of Methyltransferases; Jockmann, E., Girame, H., Steinchen, W., Kind, K., Bange, G., Tittmann, K., Müller, M., Feixas, F., Garcia-Borràs, M., Andexer, J. N.; ACS Catal. 2025
Manuscript
2024
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Non-canonical C16 Homoterpene Biosynthesis Widespread in Actinobacteria; Reuter, T., Dieminger, L., Steidle, S., Zoller, K., Holocher, M., Zhou, L., Hanauska, D., Racz, K., Barra, L.; Angew. Chem. Int. Ed. 2024.
Manuscript - Enzymatic synthesis of S-adenosyl-L-homocysteine and its nucleoside analogs from racemic homocysteine thiolactone; Wen, X.,Leopold, V., Seebeck, F. P.; Chem. Sci. 2024.
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Structures and protein engineering of the α-keto acid C-methyltransferases SgvM and MrsA for rational substrate transfer; Sommer-Kamann, C., Breiltgens, J., Zou, Z., Gerhardt, S., Saleem-Batcha, R., Kemper, F., Einsle, O., Andexer, J. N., Müller, M.; ChemBioChem 2024.Manuscript
- Efficient Transferase Engineering for SAM Analog Synthesis from Iodoalkanes; Schülke, K. H., Fröse, J. S., Klein, A., Garcia-Borràs, M., Hammer, S. C.; ChemBioChem 2024.
Manuscript and Research Data
Important Preparatory Work (until 2023)
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Biomimetic S-Adenosylmethionine Regeneration Starting from Multiple Byproducts Enables Biocatalytic Alkylation with Radical SAM Enzymes;
Gericke, L., Mhaindarkar, D., Karst, L. C., Jahn, S., Kuge, M., Mohr, M. K. F., Gagsteiger, J., Cornelissen, N. V., Wen, X., Mordhorst, S., Jessen, H. J., Rentmeister, A., Seebeck, F. P., Layer, G., Lönarz, C., Andexer, J.; ChemBioChem 2023, 24 (9), e202300133
Manuscript
- Expanding the Substrate Scope of N- and O-Methyltransferases from Plants for Chemoselective Alkylation;
Jockmann, E., Subrizi, F., Mohr, M. K. F., Carter, E. M., Hebecker, P. M., Popadić, D., Hailes, H. C., Andexer, J. N.; ChemCatChem 2023, 15 (22), e20230930.
Manuscript
- EnzymeML: Seamless Data Flow and Modeling of Enzymatic Data;
Lauterbach, S., Dienhart, H., Range, J., Malzacher, S., Spöring, J. D., Rother, D., Pinto, M. F., Martins, P., Lagerman, C. E., Bommarius, A. S., Høst, A. V., Woodley, J. M., Ngubane, S., Kudanga, T., Bergmann, F. T., Rohwer, J. M., Iglezakis, D., Weidemann, A., Wittig, U., Kettner, C., Swainston, N., Schnell, S., Pleiss; J. Nat. Methods 2023, 20 (3), 400-402.
Manuscript - Enzymatic Synthesis of l-Methionine Analogues and Application in a Methyltransferase Catalysed Alkylation Cascade;
Mohr, M. K. F., Saleem-Batcha, R., Cornelissen, N., Andexer, J. N.; Chem. Eur. J. 2023, 29 (46), e202301503.
Manuscript -
Noncanonical Functions of Enzyme Cofactors as Building Blocks in Natural Product Biosynthesis;
Barra, L., Awakawa, T., Abe, I.; JACS Au 2022, 2, 9, 1950–1963.
Manuscript
- EnzymeML—a Data Exchange Format for Biocatalysis and Enzymology;
Range, J., Halupczok, C., Lohmann, J., Swainston, N., Kettner, C., Bergmann, F. T., Weidemann, A., Wittig, U., Schnell, S., Pleiss, J.; FEBS J. 2022, 289 (19), 5864–5874.
Manuscript
- Biocatalytic One-Carbon Transfer - A Review;
Germer, P., Andexer, J. N., Müller, M.; Synthesis 2022, 54 (20), 4401-4425
Manuscript - Selective Biocatalytic N-Methylation of Unsaturated Heterocycles;
Ospina, F., Schülke, K. H., Soler, J., Klein, A., Prosenc, B., Garcia-Borràs, M., Hammer, S. C.; Angew. Chem. Int. Ed. 2022, 61, e202213056.
Manuscript - β-NAD as a building block in natural product biosynthesis;
Barra, L., Awakawa, T., Shirai, K., Hu, Z., Bashiri, G., Abe, I.; Nature 2021, 600, 754–758.
Manuscript - Engineered Enzymes Enable Selective N-Alkylation of Pyrazoles With Simple Haloalkanes;
Bengel, L. L., Aberle, B., Egler-Kemmerer, A.-N., Kienzle, S., Hauer, B., Hammer, S. C.; Angew. Chem. Int. Ed. 2021, 60, 5554–5560.
Manuscript - Progress Curve Analysis within BioCatNet: Comparing Kinetic Models for Enzyme-Catalyzed Self-Ligation;
Buchholz, P. C. F., Ohs, R., Spiess, A. C., Pleiss, J.; Biotechnol. J. 2019, 14 (3), 1–8.
Manuscript - Asymmetric C-Alkylation by the S-Adenosylmethionine-Dependent Methyltransferase SgvM;
Sommer-Kamann, C.; Fries, A.; Mordhorst, S.; Andexer J. N.; Müller, M.; Angew. Chem. Int. Ed.; 2017, 56, 4033–4036.
